The Library / Protocols

Training days

Where physical load fits in a three layer day, with the honest ranking of what helps: sleep and caffeine first, botanicals after.

TypeProtocolBasisEvidence + practiceSources9 primary

In short

On a training day the two interventions with the strongest evidence are not exotic: caffeine at 3 to 6 mg per kilogram about an hour before load, taken early enough to clear before bed, and more sleep. The system's botanicals have real but smaller signals, several of them at doses above what this formulation carries, and this page says which is which. Recovery is where a training day is actually won, and that is the layer this system is built around.

A supplement brand writing about training has one honest obligation: to rank its own ingredients below the things that actually move performance. Here is that ranking.

01The honest ranking

Caffeine is the best evidenced legal performance aid in existence. The International Society of Sports Nutrition's position stand puts the effective range at 3 to 6 mg per kilogram of body weight, taken around 60 minutes before exercise, with benefits across endurance, strength and power.1 The second lever is sleep: when college basketball players extended sleep toward ten hours for several weeks, sprint times fell and shooting accuracy rose by around nine percent, an uncontrolled study but a large signal.2 The biggest levers are behavioural; the system's job is everything around them.

02What the botanicals actually show

Read against that standard, the formulation's training relevant ingredients sort honestly. Ashwagandha has the strongest case: a meta-analysis found likely improvements in strength, VO2max, fatigue and recovery, in trials mostly at 300 to 600 mg of root extract, a range this formulation's evening 300 mg sits inside.3 Rhodiola shows small pooled improvements in endurance markers, but the largest effects cluster at daily doses above 600 mg, several times the 133 mg standardised dose here, which is dosed for its stress and fatigue evidence, not as an ergogenic.4 Cordyceps shows signals on VO2peak and ventilatory threshold in a 2025 meta-analysis, with trials at 2 to 3 grams a day against 300 mg here, and with most included studies favourable enough to warrant a publication bias caveat.5 Its place here is the daytime layer's continuity job, not a race day boost. And tyrosine helps cognition under load, not the legs: it supports decision making when stress is depleting catecholamines, while attempts to show direct exercise performance gains have largely failed to replicate.67 Magnesium is the quiet exception: it carries authorised EU claims for normal muscle function and the reduction of tiredness and fatigue, which is exactly the register it earns.

03The day, structured

The structure follows the evidence. Caffeine early, sized to body weight, timed so the cutoff arithmetic from the caffeine protocol still holds; LAYER ONE with it, since tyrosine's job is the head under load. Train where the day allows; the mid afternoon dip is a real but workable window, covered in the crash protocol. Then protect the part that pays: the evening. LAYER THREE, the wind-down, and a bedtime that lets sleep do the adaptation is where the session becomes progress.

04What this system is not

The honest limit

LAYER ZERO is not a pre workout and does not pretend to be one. It contains no caffeine, no creatine, and its botanical doses are set for daily, sustainable use rather than acute performance peaks. The performance layer of a training day belongs to caffeine, programming and sleep. What it offers a training life is the baseline: steadier days, supported recovery, and a formulation that does not have to be cycled off.

05The research horizon

The training-and-sleep question got its biggest dataset ever in 2025: 4 million person-nights of wearable data across 14,689 people, published in Nature Communications. The finding refines the old advice usefully: exercise ending four or more hours before sleep did not disturb it at all, while high-strain sessions inside that window delayed sleep onset by up to 80 minutes and cut duration by around 43 minutes.8 The rule is intensity times proximity, not a curfew on evening training, and the protocol's scheduling guidance reads exactly that way. The other direction of the loop is equally quantified now: a 2024 meta-analysis of 27 studies confirmed that lost sleep measurably degrades next-day athletic performance, with late-night partial deprivation the most damaging pattern.9 Training loads the system, sleep pays the loan, and the data on both sides of that sentence keeps getting bigger. Structure the day around it and the loop compounds in your favour.

06Where the layers sit

In the system

The three layers keep their usual places on a training day: morning focus before load, daytime resilience through it, evening recovery after it. For the two ingredients with the most direct training relevance, see ashwagandha and magnesium. To place your own layers, the protocol builder takes about three minutes. Build your protocol →

Sources

01
Guest NS, et al. International Society of Sports Nutrition position stand: caffeine and exercise performance. Journal of the International Society of Sports Nutrition, 2021. 3 to 6 mg per kg, about 60 minutes pre exercise.
Strong · position stand
pubmed.ncbi.nlm.nih.gov · 33388079
02
Mah CD, et al. The effects of sleep extension on the athletic performance of collegiate basketball players. Sleep, 2011. Faster sprints, about 9 percent better shooting; small and uncontrolled.
Limited · uncontrolled
pubmed.ncbi.nlm.nih.gov · 21731144
03
Bonilla DA, et al. Effects of ashwagandha on physical performance: systematic review and Bayesian meta-analysis. Journal of Functional Morphology and Kinesiology, 2021. Likely improvements in strength, VO2max, fatigue and recovery; trials mostly 300 to 600 mg.
Moderate · meta-analysis
pubmed.ncbi.nlm.nih.gov · 33670194
04
Wang X, et al. The effect of Rhodiola rosea supplementation on endurance performance and related biomarkers: a systematic review and meta-analysis. Frontiers in Nutrition, 2025. Small pooled effects on VO2max and time to exhaustion; largest at doses above 600 mg per day.
Moderate · meta-analysis
pubmed.ncbi.nlm.nih.gov · 41080184
05
Shu MY, et al. Effects of fungal supplementation on endurance, immune function and hematological profiles in adult athletes: a systematic review and meta-analysis. Frontiers in Nutrition, 2025. VO2peak and ventilatory threshold improved; publication bias caveat; trials at 2 to 3 g per day.
Moderate · meta-analysis
doi.org · 10.3389/fnut.2025.1670416
06
Jongkees BJ, et al. Effect of tyrosine supplementation on clinical and healthy populations under stress or cognitive demands: a review. Journal of Psychiatric Research, 2015. Benefit appears when demand depletes catecholamines; none at rest.
Moderate · review
pubmed.ncbi.nlm.nih.gov · 26424423
07
Tumilty L, et al. Failure of oral tyrosine supplementation to improve exercise performance in the heat. Medicine and Science in Sports and Exercise, 2014. The earlier positive finding did not replicate under self paced exercise.
Limited · replication
pubmed.ncbi.nlm.nih.gov · 24389518
08
Leota J, et al. Evening exercise timing, intensity and sleep: 4 million person-nights of wearable data. Nature Communications, 2025. No disruption when ending 4 or more hours before bed; high strain closer in delayed sleep onset.
Strong · large cohort
doi.org · 10.1038/s41467-025-58271-x
09
Gong M, et al. Sleep deprivation and athletic performance: meta-analysis of 27 studies. Nature and Science of Sleep, 2024. Pooled performance decline; late-night partial deprivation most damaging.
Strong · meta-analysis
doi.org · 10.2147/NSS.S467531

How we handle evidence. This page ranks the brand's own ingredients below caffeine and sleep because that is what the literature shows, and it names where studied doses exceed the formulation's. Educational content, not medical advice.

LAYER ZERO · The Library

A body of knowledge on daily performance: the biology of focus, resilience, and recovery, and the reasoning behind the system. Sources are primary literature; claims are graded by strength of evidence. Not medical advice.